|
週次 |
日期 |
單元主題 |
|
第2週 |
2/22 |
1. Introduction for recombinant GFP purification
2. Reagent preparation
3. Small scale culture incubation
4. Large scale culture incubation and IPTG O/N induction
Collect cultural cell |
|
第3週 |
3/1 |
1. Recombinant GFP purification by FPLC
2. Dialysis |
|
第4週 |
3/8 |
1. PAGE and western blot analysis
2. Prepare LB medium
3. Small scale culture incubation for T4 DNA ligase
Large scale culture incubation and IPTG O/N induction |
|
第5週 |
3/15 |
1. Recombinant T4 DNA ligase purification by FPLC
2. Dialysis |
|
第6週 |
3/22 |
1. Biochemistry assay for T4 DNA ligase activity
a. Plasmid restriction enzyme digestion
b. DNA clean up
c. DNA self-ligation/transformation
d. Data collection (3/23) |
|
第7週 |
3/29 |
Introduction NGS application in bioinformatics |
|
第8週 |
4/5 |
Data analysis (I): CLCbio |
|
第9週 |
4/12 |
Data analysis (II): Galaxy |
|
第10週 |
4/19 |
Data analysis (III): BLAST2GO |
|
第11週 |
4/26 |
Data analysis (IV): Contigviews |
|
第12週 |
5/3 |
Public domain database |
|
第13週 |
5/10 |
Building a ContigViews database (I) |
|
第14週 |
5/17 |
Building a ContigViews database (II) |
|
第15週 |
5/24 |
Data mining (I) |
|
第16週 |
5/31 |
Data mining (III) |
|
第17週 |
6/7 |
Data mining (III) |
|
第18週 |
6/14 |
Student report |